The cells were seeded
WebOct 29, 2024 · Single-cell suspensions were seeded into six-well plates at three different cell densities. Upon adherence, the culture medium (2 ml/well) was refreshed, and cells were … WebApr 26, 2024 · All cell morphology images were taken by a Leica Microsystems microscope. RESULTS: XN could lead to proliferation inhibition and death of C6 cells in a time- and dose-dependent manner and induce apoptosis of C6 cells through the AIF pathway.
The cells were seeded
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WebNov 1, 2024 · The cells were then seeded on to Petri dishes, welled plates, culture flasks or onto live imaging chambers with pre-warmed medium in the usual manner. Resazurin assay. To assess cell recovery, cell number was monitored using a standard resazurin assay. Briefly, cells were seeded into 96-well plates in quadruplicate, and incubated for 1 h at 37 ...
WebJan 7, 2024 · To determine the cell proliferation rate of MCF-7 wt, Control KO, Bag-1 KO, and Bag-1 OE cells, and to investigate the effect of MK-2206, cells were seeded at 5x10 4 cells/well density in 6-well plates. According to the time points (24 h, 48 h, 72 h), cells trypsinized and counted every 24 h after staining with 0.4% trypan blue dye by using a ... WebOct 7, 2013 · Cells were seeded on unpatterned and patterned (20-μm wide lines) surfaces for 24 h; they were then removed from the substrate and stained with primary and fluorescently labeled secondary antibodies. We ... Cells were incubated with blebbistatin to inhibit myosin phosphorylation, cytochalasin D to inhibit actin polymerization, Y27632 to ...
WebC2C12 cells were seeded at varying density in serum free medium in a 96-well plate and incubated overnight. Serum was added to the plate at various concentrations and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr Detection of proliferation proteins WebK562 cells were seeded at 750, 1500, 3000 or 6000 cells/well in a 384-well opaque white plate in 80μL of RPMI medium supplemented with 10% FBS and Pen/Strep that contained the RealTime-Glo™ Reagent. Luminescence was monitored every hour for 72 hours using a Tecan Infinite ® 200 Multimode Reader with Gas Control Module (37°C and 5% CO 2 ).
WebDMSCs were seeded in a 24-well plate and incubated overnight prior to transfection with P4H shRNA or an empty vector control plasmid. At 48 h following transfection, the supernatant and cells were ...
Web25 rows · The aforementioned cells were seeded onto a degradable suture material and … do all jobs require a background checkWebSentence examples for cells were seeded in from inspiring English sources. Sentence examples for. cells were seeded in. from inspiring English sources. RELATED ( 13) cells … create sharepoint site for file sharingWebMay 10, 2005 · Briefly, cells were harvested as the following: 1) For suspension cells, 1 ml of Jurkat cell suspensions was transferred to a 15 ml tube. 2) For adherent cells, supernatant (medium and floating A375 cells) were transferred to 15 ml tubes. create sharepoint shortcut on desktopWebThe cells that were seeded in E-Plate 96 and were constantly observed by measuring CI values to obtain the ideal cell number and to distinguish the suitable time point for virus infection prior to ... create sharepoint shortcut in file explorerWebMar 4, 2024 · Cell seeding is the first stage of cell attachment, and its efficiency and distribution can affect the final biological performance of the scaffold. One of the contributing factors to maximize cell seeding efficiency and consequently cell attachment is the design of the scaffold. create sharepoint online templateWebMay 31, 2024 · SVEC4-10 cells (8 × 10 3 cells per well) were seeded in collagen-coated cover slips in 24-well plates and cultured for three days. Cells were treated with EVs derived from non-infected and Mtb -infected macrophages (40μg/mL) for 4 hrs. After PBS wash, cells were fixed with 4% PFA for 15mins at room temperature. create sharepoint site for external usersWebFirst, the cells were seeded in 96-well plates with 5 × 10 3 cells/well and incubated for 24 h. Then they were washed with phosphate-buffered saline (PBS) and starved-cultured in the corresponding low-glucose Dulbecco's Modified Eagle's Medium (DMEM) containing 0.4% fetal bovine serum (FBS) for 24 h. create sharepoint site flow