2024 Ribofect cp transfection kit 166t

Ribofect cp transfection kit 166t

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Webb31 jan. 2014 · To determine the effects of miRNA-23 downregulation on NPC radioresistance, miRNA-23a mimic and mimic control (RiboBio) were transfected into the CNE2-IR cells using riboFect™ CP transfection kit (RiboBio) according to manufacturer's instructions, respectively. 48h after transfection, the expression level of IL-8 was … Webb18 nov. 2015 · UACC903 cells in 6-well plates were transfected with 100 μM siLEF-1 or negative control siRNA using a RiboFect CP Transfection Kit (166 T) (RiboBioCo. Ltd., …

GPM6A expression is suppressed in hepatocellular carcinoma …

WebbAdditionally, to silence the expression of circFAM114A2 and HHIP, two small interfering RNAs (siRNA) were synthesized by RiboBio (China). The transfection of siRNAs, miR-630 mimic, and scramble controls were performed using the riboFECT CP Transfection Kit (Ribobio, C10511-05). All siRNA sequences are shown in Table S1. Webbwherein the double-stranded RNAi agent comprises a sense strand and an antisense strand forming a double stranded region, the antisense strand comprising a region of complementari characteristics of generativity

MiR-20a-5p represses multi-drug resistance in osteosarcoma by …

Webb10 jan. 2024 · The above oligonucleotides products transfected H9c2 cells for 24 h by using the riboFECT CP Transfection Kit (RiboBio, China) as directed by the protocol of the manufacturer. Briefly, the oligonucleotides were diluted with 1× riboFECT CP buffer, then added with riboFECT CP reagent and mixed gently. Webb25 juni 2024 · The cells were transfected with pmirGLO-circTLK1 or pmirGLO-HMGB1, and miR-106a-5p mimic or control mimic by using the riboFECT™ CP Transfection Kit (RiboBio, China), followed by the analysis of luciferase activities based on the Dual-Luciferase Reporter Assay System (Promega, United States). WebbpSicoR-GSK-3β group. During transfection, 100 μL 1 X riboFECTTM CP buffer was used to dilute 3 μL pSicoR-blank, pSicroR-GSK-3β, miR-NC, and miR-224 inhibitor. After room … harper design classics

The miR-199a-3p regulates the radioresistance of esophageal cancer …

RNF6 promotes myeloma cell proliferation and survival by …

WebbriboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂，极具生物相容性，转染效率高，细胞毒性小，适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂 … Webb7 sep. 2016 · RiboFECT CP Transfection kit (166T) (RiboBio) was used for the miR-671 inhibitor and mimic. Detection of cell apoptosis by FCM The Annexin V-fluorescein … characteristics of generation z consumersWebb16 nov. 2024 · All the transfection experiments were performed using riboFECT CP transfection kit were supplied by Guangzhou Ribobio, China. Western blot and qRT–PCR assays were performed to confirm the effect of AK4 on the expression of miR–199a-3p. The sequences used in this study are as follows: si-AK4: GCCTAATGATGTCCGAGTT 5′ … characteristics of generous people

"WebbVSMCs were seeded into 6-well cell culture plates and incubated for 48 h. We used a riboFECT CP Transfection Kit (CAT No: C10511-05, RIBO, Guangzhou, China) and riboFECT CP buffer (CAT No: C10502-05, RIBO, Guangzhou, China) to perform transfection. Then, cells were subjected to transfection with siTMEM16A and siESM1 for 24 and 48 h, … " - Ribofect cp transfection kit 166t

Ribofect cp transfection kit 166t

riboFECT™ CP转染试剂 C10511-05、C10511-1报价/价格 - 广州市 …

Webb17 sep. 2024 · RAW264.7 cells were transfected with the siRNA using the riboFECT CP Transfection Kit (C10511-05) according to the manufacturer’s instructions. The efficiency of transfection was evaluated via ... After in vitro transfection with the three siRNAs, we chose RNAi-1, which had the best efficacy, for further in vitro and in vivo ... WebbRibofect Cp Transfection Kit 166 T Ribobioco, supplied by Ribobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

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WebbPrice from $9.99 to $1999.99 ribofect cp transfection kit - by Bioz Stars , 2024-04 86 / 100 stars Buy from Supplier ribofect cp transfection kit ( Thermo Fisher ) 86 Thermo Fisher … Webb5 aug. 2016 · Mimic, agomir, antagomir, and scrambled (negative control, NC) siRNA sequences as well as a riboFECT CP transfection kit were supplied by Guangzhou RiboBio, China. A GFP-tagged KIF26B overexpression construct (pReciever-M98) was purchased from Genecopia, Guangzhou, China (Catalog No.: EX-E0804-M98-5).

Webb166T 产品描述 ribo FectTM CP转染试剂是一种专门用于核酸分子的转染试剂，极具生物相容性，转染效率高，细胞毒性小，适合各种核酸分子 (如siRNA、miRNA、质粒)的转染 … WebbThe above results showed that RNF6 mediates GR polyubiquitination; to determine the effects of RNF6 on GR stability, wild-type or RING domain deletion (ΔRING) plasmids were cotransfected with a GR...

Webb4 dec. 2024 · The RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Lithuania, USA) ... (15 × 10 4 cells/well) were seeded in a 6-well plate and, after 24 h, were transfected with siRNA at 100 nM by riboFECT™ CP Transfection Kit (RiboBio) according to the manufacturer's instructions. For the stable silencing, shRNA lentivirus ... Webb2 aug. 2024 · Transfection of miR-96 mimetics and mimetic controls were performed with riboFECT CP Transfection Kit (RiboBio) according to the manufacturer’s instruction. The final concentration of...

Webb13 jan. 2024 · siRNA and plasmid transfections. The human esophageal carcinoma cells (KYSE150, KYSE450) were grown in 60-mm plates at about 30% confluence and …

Webb26 rader · 8 aug. 2024 · 英文名称： ribo FECT ™ CP Transfection 产品货号： C10511-05、C10511-1 产品规格： 166T、333T 产品产地：广州产品商标： ribobio 价格：询价保 … harper dental fort smith arWebb18 nov. 2015 · UACC903 cells were transfected with negative control siRNA (100 nM) or target gene siRNA (si LEF-1 or si MITF) (100 nM) using RiboFect CP Transfection Kit (166 T) (RiboBioCo. Ltd. Guangzhou, China) according to the manufacturer’s instructions. harper dentist carrickfergusWebb14 aug. 2024 · Melanocyte transfections were performed using riboFECT™ CP (Ribobio) in accordance with the manufacturer’s protocol. Approximately 5 μL of 20 μM miRNA … characteristics of generic prescription drugsWebbRibobio co c10511 05 ribofect cp transfection kit 166t C10511 05 Ribofect Cp Transfection Kit 166t, supplied by Ribobio co, used in various techniques. Bioz Stars … characteristics of genus mycalesisWebb2 nov. 2024 · Transfection of both ribonucleic acid reagents or plasmids mentioned above and the reporter plasmids in a Cignal Finder Pathway Reporter package (Qiagen, Hilden, Germany) was performed according to the manufacturer’s instruction. The sequences used in this study are as follows: si-SDC2: 5′-GAAACCACGACGCTGAATA-3′ hsa-miR-20a-5p characteristics of gen x workersWebb13 mars 2024 · 转染方法以riboFECT? CP Reagent 转染siRNA于24孔板，转染浓度为50nM为例，其他规格容器转染请参考表2。. 1. 接种细胞 a. 贴壁细胞：转染前一天，接种1×105细胞至含有适量完全培养基的24孔板培养孔中，使转染时的细胞密度能够达到50~80%。. 注：1）不同细胞生长速度不 ... characteristics of genius mindWebb14 okt. 2014 · A, The IC 50 dosed drug-triggered cell death of 5637 cells transfected by miR-193a-3p mimic (3 PM) or the gene specific siRNAs versus the negative control (NC). B, The levels by qRT-PCR of both LOXL4 and SRSF2 mRNAs in the 3 PM, or siRNAs (single or in combination) transfected 5637 cells and the 3PA transfected H-bc cells versus the NC. harper design projects