WebMay 5, 2024 · In uninfected cells expressing GFP-FIP2, the fluorescent pattern was similar to that of GFP-FIP1C, and upon infection, it also delocalized to the PV (Figure 2Ab). Compared to the GFP-FIP1C and GFP-FIP2, the fluorescent signal of GFP-FIP5 was predominantly centralized in the juxtanuclear region of uninfected cells (Figure 2Ac). … WebSep 1, 2016 · TIRF microscopy and live cell imaging will be pursued to fully define the steps in FIP1C-Env trafficking to the particle assembly site. In Aim 2, the contribution of the FIP1C-Rab14 trafficking pathway to polarization of HIV structural components and to cell-cell transmission of virus will be addressed. Experiments in this aim will also ...
Cureus Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting …
WebApr 11, 2013 · It appears that FIP1c and Rab14 grab this tail when the viral particles are being assembled, Spearman says. “It has been a mystery for many years how HIV controls incorporation of the envelope protein into the virion, and we have worked the past four years to figure this out,” he says. WebAcademic Departments. Back; Academic Departments Alabama College of Osteopathic ... Annals of Simulation 13名党员
Rab11-FIP1C and Rab14 direct plasma membrane sorting and …
WebMay 1, 2016 · In addition to its role in regulating epithelial polarity, there is an increasing body of evidence implicating FIP1C in cancer progression. For example, FIP1C-mediated recycling of the integrin α5β1 is activated by expression of mutant forms of p53 resulting in enhanced cancer cell invasion ().In 10% of human breast cancers, amplification and … Webtion of FIP1C, FIP2, and FIP5 to the periphery of A431 cells,17 and diacylgylcerol kinase activity is required for translocation of FIP1C to pseudopods of A2780 cells.18 All these data point to an inherent role for phospholipid regulation of Rab11 and Rab11-FIP-dependent membrane recycling. Phospholipids make up approximately 70% of the lipid com - WebNov 10, 2024 · To investigate the role of FIP1C in relevant cell types—human T-cell lines and primary CD4 ⁺ T cells—we used CRISPR-Cas9 to knock out FIP1C expression and … 13名代表的平均年龄